1. MCE Kits
  2. Protein A/G Magnetic Beads

Protein A/G Magnetic Beads 

Cat. No.: HY-K0202
Manual SDS

The MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

Protein A/G Magnetic Beads

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Price Stock Quantity
Free Sample   Apply now  
1  mL ¥455 In-stock
5  mL ¥1950 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

The MCE Protein A/G Magnetic Beads are typically used for isolating antibodies from serum, cell culture supernatant or ascites and for immunoprecipitation and co-immunoprecipitation of antigens from cell or tissue extracts. Protein A/G Magnetic Beads contain a recombinant Protein A/G that combines the IgG binding domains of both Protein A and Protein G.

During immunoprecipitation, only a small amount of magnetic beads are needed, and the non-specific binding is low.

•   Convenient and time saving.

•   Low non-specific binding.

•   Minimal sample loss.

•   Antibody binding capacity up to 0。5-0。8 mg/mL。

•   Stable, one bottle solution.

Publications
Storage

Stored at 4°C, and is stable for up to 2 years。

Do not centrifuge, dry or freeze the magnetic beads。

Protocol

1.   Preparation of Magnetic Beads

1。1   Resuspend the Magnetic Beads in the vial (tilt and rotate for 2 minutes or gently pipette for 10 times)。

1.2   Transfer 25-50 μL of Protein A/G Magnetic Beads into a 1.5 mL tube (Transfer amount may be adjusted as required).

1。3   Add 400 μL of binding/wash buffer to the beads and gently pipette to mix。 Place the tube into a magnetic stand to collect the beads against the side of the tube (Hereinafter referred to as magnetic separation)。 Remove and discard the supernatant。 Repeat this step for 2 times。

2.   Binding of Antibody

2.1   Dilute antibody (Ab) to the final concentration of 5-50 μg/mL with binding/wash buffer. The optimal amount of Ab may be adjusted as required.

2.2   Add 400 μL of diluted Ab to the Protein A/G Magnetic Beads. Rotate tube for 30 minutes at room temperature or 2 hours at 4°C.

2。3   Perform magnetic separation。 Transfer the supernatant into a new tube for further analysis, if desired。 The supernatant is the non-binding fraction。

2.4   Add 400 μL of binding/wash buffer to the beads and gently pipette to mix. Place the tube into a magnetic stand to collect the beads against the side of the tube. Remove and discard the supernatant. Repeat this step for 4 times.

3.   Immunoprecipitation of Target Antigen

3.1   Remove the tubes from the magnetic separator and add your sample containing the antigen (Ag) (typically 5-50 μg in 400 μL binding/wash buffer) and gently pipette to resuspend the Protein A/G Magnetic Beads-Ab complex.

3。2   Incubate with rotation for 30 minutes at room temperature or 2 hours at 4°C to allow Ag to bind to the Protein A/G Magnetic Beads-Ab complex。

3。3   Perform magnetic separation。 Remove and discard the supernatant。

3.4   Wash the Magbeads-Ab-Ag complex 5 times using 400 μL binding/wash buffer for each wash. Perform magnetic separation between each wash, remove supernatant and resuspend by gentle pipetting.

3。5   Resuspend the Protein A/G Magnetic Beads-Ab-Ag complex in 400 μL binding/wash buffer and transfer the bead suspension into a clean tube。 This is recommended to avoid co-elution of the proteins bound to the tube wall。

4.   Elution

This is a non-denaturation elution method。

4。1   Perform magnetic separation and remove the supernatant。 Add 400 μL of binding/wash buffer into the tube and rotate for 5 minutes。 Perform magnetic separation for 1 minute and remove the supernatant。 Then add 25-50 μL elution buffer into the tube with magnetic beads-Ab-Ag complex, rotate for 5 minutes。

4。2   Perform magnetic separation, collect the supernatant。

4.3   The final solution can be used as samples for denaturing SDS-PAGE. Or the elution can be adjusted to neutral pH with neutralization buffer immediately and used for further analysis.

Components
Components HY-K0202-1 mL HY-K0202-5 mL
Protein A/G Magnetic Beads 1 mL 1 mL × 5
Documentation

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Protein A/G Magnetic Beads
目录号:
HY-K0202
需求量:
北京赛车论坛 秒速时时彩彩票 北京赛车开奖视频直播 秒速时时彩 福利彩票北京赛车 北京赛车营业时间 北京赛车官方开奖直播 秒速时时彩 秒速时时彩 北京赛车官方开奖直播