LF3 

目录号: HY-101486 纯度: 98.58%
产品使用指南

LF3是β-Catenin/TCF4相互作用的拮抗剂,具有抗肿瘤活性。IC50值为1.65 μM。

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LF3 Chemical Structure

LF3 Chemical Structure

CAS No. : 664969-54-4

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Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥660 In-stock
5 mg ¥600 In-stock
10 mg ¥780 In-stock
25 mg ¥1750 In-stock
50 mg ¥2800 In-stock
100 mg ¥5200 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

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Description

LF3 is an antagonist of the β-Catenin/TCF4 interaction with antitumor activity; has an IC50 of 1.65 μM.

IC50 & Target

IC50: 1.65 μM (β-Catenin/TCF4, AlphaScreen), 1.82 μM (β-Catenin/TCF4, ELISA)[1]

In Vitro

LF3 inhibits Wnt/β-catenin signals in cells with exogenous reporters and in colon cancer cells with endogenously high Wnt activity. LF3 also suppresses features of cancer cells related to Wnt signaling, including high cell motility, cell-cycle progression, and the overexpression of Wnt target genes. However, LF3 does not cause cell death or interfere with cadherin-mediated cell-cell adhesion. Remarkably, the self-renewal capacity of cancer stem cells is blocked by LF3 in concentration-dependent manners[1].

In Vivo

LF3 reduces tumor growth and induces differentiation in a mouse xenograft model of colon cancer. Tumor growth is significantly reduced when mice with GFPhigh cells are treated with LF3 at 50 mg/kg. LF3 treatment does not disturb the normal histology of the gut of mice[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 32 mg/mL (76.82 mM)

* "≥" means soluble, but saturation unknown。

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4006 mL 12.0031 mL 24.0061 mL
5 mM 0.4801 mL 2.4006 mL 4.8012 mL
10 mM 0.2401 mL 1.2003 mL 2.4006 mL
*Please refer to the solubility information to select the appropriate solvent.
References
  • [1].

Cell Assay
[1]

LF3 is dissolved in DMSO to a concentration of 50 mM and diluted with culture medium. Two colon cancer cell lines (HCT116 and HT29) and a breast cancer cell line (MCF7) are treated with LF3 (0, 30, 60 μM) for 24 hours and labeled with BrdUrd for 4 to 5 hours to detect proliferating cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: Unsorted GFPlow and GFPhigh SW480 cells are subcutaneously injected into the back skin of NOD/SCID mice. Tumor growth is monitored over a period of 45 days. For therapy, LF3 is administered i.v. at 50 mg/kg body weight for three rounds over 5 consecutive days, with 2-day breaks[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • [1]。

Molecular Weight

416。56

Formula

C₂₀H₂₄N₄O₂S₂

CAS No.

664969-54-4

SMILES

S=C(N1CCN(C/C=C/C2=CC=CC=C2)CC1)NC3=CC=C(S(=O)(N)=O)C=C3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

  • [1].

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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产品名称:
LF3
目录号:
HY-101486
需求量:

LF3

Cat. No.: HY-101486

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