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Cycloheximide (Synonyms: Naramycin A; Actidione; CHX)

目录号: HY-12320 纯度: 99.45%
产品使用指南

Cycloheximide (Naramycin A) 是真核生物蛋白质合成的抑制剂,抑制体内蛋白质合成和RNA合成的 IC50 值分别为532.5 nM 和 2880 nM。

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Cycloheximide Chemical Structure

Cycloheximide Chemical Structure

CAS No. : 66-81-9

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Customer Review

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的科研文献

    Cycloheximide purchased from MCE. Usage Cited in:

    Nrf2 and Keap1 degradation in NIH-3T3 cells when protein synthesis is inhibited by 50 μM Cycloheximide (n=3).

    Cycloheximide purchased from MCE. Usage Cited in:

    AGS cells transfected with HA-tagged SQSTM1 plasmid, are treated with 25 μg/mL Cycloheximide (CHX) over a 240-min time period or treated with 100 μg/mL PPI for 48 h in pH 7.4 conditions, and then followed by 25 μg/mL CHX over a 240-min time period.

    Cycloheximide purchased from MCE. Usage Cited in:

    The expression levels of apoptosis-associated proteins after treatment with morusin or tumor necrosis factor-alpha (TNF-α)/Cycloheximide (CHX). A2780 cells are incubated with the indicated concentrations of morusin or TNF-α (50 ng/mL)/CHX (50 μM) for 24 h. GAPDH is used as a loading control in western blot analysis.

    Cycloheximide purchased from MCE. Usage Cited in:

    The expression levels of apoptosis-associated proteins after treatment with morusin or tumor necrosis factor-alpha (TNF-α)/Cycloheximide (CHX). SKOV-3 cells are incubated with the indicated concentrations of morusin or TNF-α (50 ng/mL)/CHX (50 μM) for 24 h. GAPDH is used as a loading control in western blot analysis.

    Cycloheximide purchased from MCE. Usage Cited in:

    PIG3 silencing does not affect the degradation of HIF-1α protein under hypoxia. Forty-eight hours after transfection with PIG3-siRNA or negative control siRNA, CAKI cells are pretreated under hypoxia for 4 h followed by treatment with 100 μg/mL Cycloheximide (CHX) to block protein synthesis for the indicated times. The mean values from two experiments are connected by the lines. Protein levels are analyzed by Immunoblotting, GAPDH is employed a loading control. All the experiments above are cond

    Cycloheximide purchased from MCE. Usage Cited in:

    SR-B1 protein is degraded by proteasome pathway. (A) SR-B1 is degraded in a CHX dose-dependent manner in CHO-K1 cells. (B) Time course of SR-B1 degradation in CHO-K1 cells treated with CHX.

    Cycloheximide purchased from MCE. Usage Cited in:

    Cells are treated with CHX in the presence or absence of CQ and results show that the decrease NDRG1 observed after CHX treatment is partially recovered upon co-treatment with CHX and CQ.

    Cycloheximide purchased from MCE. Usage Cited in:

    Immunoblotting analysis of HOXB13 in MCF-7 cells time-dependently treated with 100 μg/mL Cycloheximide (CHX) after being transiently transfected with the indicated plasmids.

    Cycloheximide purchased from MCE. Usage Cited in:

    (A) PK15 cells are transfected with Flag-ORF4 for 24 h, and then treated with CHX for 0, 3, 6, 9, 12, and 15 h respectively. (B) PK15 cells are transfected with Flag-ORF4 for 24 h, and then exposed to both CHX and MG132 for 9h.

    Cycloheximide purchased from MCE. Usage Cited in:

    Protein biosynthesis in MCF-7 cells is blocked with 100 μg/mL of Cycloheximide (CHX). MRTF-A protein levels in cells with or without LiCl co-treatment are measured with Western-blot at different time points.

    Cycloheximide purchased from MCE. Usage Cited in:

    MCF-7 cells transfected with pIRES2-EGFP-3Flag/BRD7 or pIRES2-EGFP for 24 h and treated with CoCl2 (150 μM) for 24 h. Then the cells are incubated with 50 μg/mL Cycloheximide (CHX) for the indicated periods of time (0, 1, 3, 6 h). Lysates are harvested from the cells and analyzed by western blotting.

    Cycloheximide purchased from MCE. Usage Cited in:

    Protein biosynthesis in MCF-7 cells is blocked with 100 μg/mL of Cycloheximide (CHX). MRTF-A protein levels in cells with or without LiCl co-treatment are measured with Western-blot at different time points.

    Cycloheximide purchased from MCE. Usage Cited in:

    Protein synthesis is inhibited by Cycloheximide (Cyclo).

    Cycloheximide purchased from MCE. Usage Cited in:

    pVHL interacts with NEK8 and promotes NEK8 degradation through the proteasome ubiquitination signaling pathway.

    Cycloheximide purchased from MCE. Usage Cited in:

    Representative western blots show TP53 expression in untreated and Sunitinib-treated primary colon cancer cells.

    Cycloheximide purchased from MCE. Usage Cited in:

    Cycloheximide (CHX) assay is performed to investigate the effects of CIRBP on HIF-1α protein stability.

    Cycloheximide purchased from MCE. Usage Cited in:

    Western analysis of NEK1 and VHL protein expression in the treatment such as CHX.

    Cycloheximide purchased from MCE. Usage Cited in:

    A549 cells are mock infected or infected with H1N1 at an MOI of 1. At 22 h p.i., cells are treated with DMOG for 2 h and then exposed to 50 μg/mL CHX. Cells are harvested at indicated time points after CHX addition and HIF-1α levels were measured by western blotting.

    Cycloheximide purchased from MCE. Usage Cited in:

    FBX022 ubiquitinates p21 via the F-box domain HLF and Hep3B are treated with CHX (10 μM), collected at the indicated time points, and immunoblotted for FBXO22, p21 and GAPDH. Quantification of the p21 levels relative to GAPDH expression is shown.

    Cycloheximide purchased from MCE. Usage Cited in:

    Western blot analysis of 97-L and 97-H cells incubated with 100 μM CHX in the absence or presence of JQ1.
    • 生物活性

    • 实验参考方法

    • 技术信息

    • 纯度 & 产品资料

    • 参考文献

    Description

    Cycloheximide (Naramycin A) is an eukaryote protein synthesis inhibitor, with IC50s of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively.

    IC50 & Target

    IC50: 532.5 nM (protein synthesis), 2.88 μM (RNA synthesis)[1]

    In Vitro

    Cycloheximide (CHX) is the most common laboratory reagent used to inhibit protein synthesis. Cycloheximide has been shown to block the elongation phase of eukaryotic translation. Cycloheximide binds the ribosome and inhibits eEF2-mediated translocation. Surprisingly, Cycloheximide allows one complete translocation cycle to proceed before halting any further elongation. Cycloheximide has been speculated that Cycloheximide requires an E-site bound deacylated tRNA for activity[1].

    In Vivo

    The mice receive Cycloheximide injections at 30, 60, or 120 mg/kg prior to training with a 200 µA shock. There is a significant effect of Cycloheximide on latencies on the memory test trials (P<0.001). In saline controls, this shock level results in latencies on the test trial that are significantly higher than those at training. Injections of the lowest dose of Cycloheximide tested, 30 mg/kg, results in latencies on the test trial that are significantly higher than those seen in the saline control group. Mice receiving either of the two higher doses of Cycloheximide has latencies on the test trial that are comparable to those of the saline group, i.e., the higher doses neither enhanced nor impaired memory under these conditions, resulting in an inverted-U dose-response curve for Cycloheximide enhancement of memory[2]. Infarct volume, as measured by morphometric analysis of infarct areas with triphenyl tetrazolium chloride (TTC), is significantly reduced by 92% and 61% when Cycloheximide is given 0 or 6 hr after HI respectively, but shows an insignificant trend in infarct reduction if Cycloheximide is administered 12 hr after hypoxia-ischemia (HI) compared to the HI control group, and no protective effect is observed when administration is delayed until 24 hr after HI[3].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33 mg/mL (117.29 mM)

    H2O : 20 mg/mL (71.09 mM; Need ultrasonic and warming)

    * "≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5543 mL 17.7715 mL 35.5429 mL
    5 mM 0.7109 mL 3.5543 mL 7.1086 mL
    10 mM 0.3554 mL 1.7771 mL 3.5543 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (8.89 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.5 mg/mL (8.89 mM); Clear solution; Need warming

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (8.89 mM); Clear solution

    References
    • [1].

      [2]。

      [3].

    Cell Assay
    [1]

    To test cell proliferation, 3000-5000 cells (HeLa, HTB1 and HEK 293T cells; Jurkat, BT 474, HCC 1395, HCC 1937, HCC 2218 and MDA MB231 cells; MCF 10A) per well are plated in a 96-well plate and allowed to adhere overnight. Cycloheximide dissolved in DMSO at the indicated concentrations (0.1 nM-1000μM) are then added and cells are incubated for a further 24 h. [3H]-thymidine is added at 1 μCi per well and incubation is continued for an additional 7 h. Cells are washed twice with PBS and then trypsinized before they are collected with a Tomtec harvester and bound to GF/C filter mats. Thymidine uptake is then measured by scintillation counting[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2][3]

    Mice[2]
    Male ICR mice (approximately 2 months old) are used in this experiment. Cycloheximide is administered IP at concentrations of 0 (saline controls), 30, 60, or 120 mg/kg. Cycloheximide injections are administered 30 min prior to training. The 120 mg/kg dose is commonly used to study amnesia in mice. Note that amnestic Cycloheximide doses are much lower in rats (1-3 mg/kg) than in mice, consistent with a similar difference in LD50s for rats and mice. Cycloheximide doses of 120-150 mg/kg result in approximately 95% inhibition of brain protein synthesis as measured 30-60 min after injection; the dose of 30 mg/kg produces approximately 80% inhibition of brain protein synthesis.
    Rats[3]
    Unilateral carotid artery ligation is performed in 7-day old Sprague Dawley rat pups under methoxyflurane anesthesia. The neck is incised in the midline, and the right common carotid artery is permanently ligated with 4-0 silk. Total time of surgery in each animal never exceeded 5 min. Following surgery, rats are returned to their mother for recovery and feeding for 2 hr. The pups are then exposed to a 100 min-period of hypoxia (8% O2, 92% N2) by placing them in an airtight chamber partially submerged in a temperature controlled water bath to maintain the ambient temperature inside the chamber at a constant 36°C. In the HI with Cycloheximide treatment group, the rat pups receive an intraperitoneal injection of Cycloheximide at a dose of 0.6 mg/kg at 0, 6, 12 or 24 hr of recovery, and an equal volume of normal saline is given to a HI control group. Then, the rat pups are returned to their dam until sacrifice; the whole brain tissue is obtained under deep pentobarbital anesthesia (60 mg/kg, intraperitoneal) for flow cytometry and triphenyl tetrazolium chloride (TTC) at 48 and 72 hr after HI, respectively.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    • [1].

      [2].

      [3]。

    Molecular Weight

    281.35

    Formula

    C₁₅H₂₃NO₄

    CAS No.

    66-81-9

    SMILES

    O=C(CC(C[C@H]([C@H]1C([C@@H](C)C[C@H](C)C1)=O)O)C2)NC2=O

    Storage

    4°C, protect from light

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.45%

    • [1]。

      [2].

      [3].

    • 摩尔计算器

    • 稀释计算器

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
    C1   V1   C2   V2

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    产品名称:
    Cycloheximide
    目录号:
    HY-12320
    需求量:

    Cycloheximide

    Cat. No.: HY-12320

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